| 货号 | 8161S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | eIF3J (D21G7) XP® Rabbit mAb recognizes endogenous levels of total eIF3J protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Translation initiation requires a set of factors to facilitate the association of the 40S ribosomal subunit with mRNA. The eIF4F complex, consisting of eIF4E, eIF4A, and eIF4G, binds to the 5 cap structure of mRNA. eIF4F and eIF4B unwind the secondary structure of mRNA at its 5 untranslated region. The 40S ribosomal subunit, along with some initiation factors including eIF3, then binds to the 5 mRNA cap and searches along the mRNA for the initiation codon. eIF3 is a large translation initiation complex with 10 to 13 different subunits. eIF3A, eIF3B, eIF3C, eIF3E, eIF3F, and eIF3H are the core subunits critical for the function of this complex. eIF3 physically interacts with eIF4G, which may be responsible for the association of the 40S ribosomal subunit with mRNA (1). eIF3 also stabilizes the binding of Met-tRNAf.eIF2.GTP to the 40S ribosomal subunit and helps keep the integrity of the resulting complex upon addition of the 60S ribosomal subunit (2). Studies have shown that mTOR interacts with eIF3 directly (3,4). When cells are stimulated by hormones or mitogenic signals, mTOR binds to the eIF3 complex and phosphorylates S6K1 (3). This process results in the dissociation of S6K1 from eIF3 and S6K1 activation. The activated S6K1 then phosphorylates its downstream targets including ribosomal protein S6 and eIF4B, resulting in stimulation of translation. Further findings demonstrated that activated mTOR signaling induces the association of eIF3 with eIF4G upon stimulation with insulin (3). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
Confocal immunofluorescent analysis of HeLa cells, mock transfected or transfected with eIF3J-specific siRNA, using eIF3J (D21G7) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光术分别检测使用空白对照物转染或eIF3J-specific siRNA 转染的HeLa细胞，使用的抗体为 eIF3J (D21G7) XP® Rabbit mAb兔单抗 (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。 | |
Western blot analysis of extracts from various cell lines using eIF3J (D21G7) XP® Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为eIF3J (D21G7) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with eIF3J-specific siRNA (+), using eIF3J (D21G7) XP® Rabbit mAb. Western blot方法分别检测空白对照物转染（-）或eIF3J-specific siRNA (+)转染的HeLa细胞提取物，使用的抗体为 eIF3J (D21G7) XP® Rabbit mAb兔单抗。 |
