| 货号 | 12156S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | <p>Echinoderm microtubule-associated protein-like 4 (EML4) is a 120 kDa microtubule-associated WD-repeat protein of the echinoderm microtubule-associated protein family (1). The expression of EML4 is necessary for correct intracellular microtubule network formation (2). EML4 protein expression is upregulated during mitosis and downregulated during the remaining cell cycle (1). During mitosis, EML4 is heavily phosphorylated and is associated with the mitotic spindle (2). The amino-terminal segment of EML4 is essential for microtubule association and function.</p>棘皮动物微管样蛋白4（EML4）是棘皮动物微管样蛋白家族一个120kDa的微管相关的WD重复蛋白（1）。EML4的表达对于正确的细胞内微管网络形成是必须的（2）。有丝分裂时EML4表达上升，在静息细胞时期表达下降（1）。有丝分裂时，EML4被深度磷酸化且和有丝分裂纺锤体结合（2）。EML4的氨基末端序列对于微管的结合和功能是必须的。 |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from various cell lines using EML4 (D7Y8F) XP® Rabbit mAb.使用EML4 (D7Y8F) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of EML4 from 293 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or EML4 (D7Y8F) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EML4 (D7Y8F) XP® Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或EML4 (D7Y8F) XP® Rabbit mAb (lane 3)对293细胞提取物中的EML4进行免疫沉淀实验。Lane1是10%上样量。 | |
Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® EML4 siRNA (right), using EML4 (D7Y8F) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).模拟转染(左)或转染了SignalSilence® EML4 siRNA (右)的HeLa细胞，使用EML4 (D7Y8F) XP® Rabbit mAb (绿色)进行激光共聚焦荧光分析。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® EML4 siRNA (+), using EML4 (D7Y8F) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The EML4 (D7Y8F) XP® Rabbit mAb confirms silencing of EML4 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.对转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568（-），或SignalSilence® EML4 siRNA（+）的HeLa细胞提取物，使用EML4 (D7Y8F) XP® Rabbit mAb (上)或β-Actin (D6A8) Rabbit mAb #8457（下）进行Western blot分析。EML4 (D7Y8F) XP® Rabbit mAb用于确认EML4的敲除，β-Actin (D6A8) Rabbit mAb用作上样对照。 |
