| 货号 | 8562S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | FGF Receptor 4 (D3B12) XP(R) Rabbit mAb detects endogeneous levels of total FGF receptor 4 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 95, 125 |
Western blot analysis of extracts from various cell lines using FGF Receptor 4 (D3B12) XP® Rabbit mAb. (Lower molecular weight degradation products of FGF receptor 4 are present in some cell lines.)Western blot检测各种细胞系提取物，使用的抗体为FGF Receptor 4 (D3B12) XP® Rabbit mAb。（在一些细胞系中FGFR4存在低分子量降解产物） | |
Confocal immunofluorescent analysis of Hep G2 (left) and HeLa (right) cells using FGF Receptor 4 (D3B12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测Hep G2 (左)和HeLa（右）细胞，使用抗体为：FGF Receptor 4 (D3B12) XP® Rabbit mAb（绿色）。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 |
