| 货号 | 5436S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331 Ser704), Cyclin/Cdk (Thr600, Thr611, Thr672), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-7). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (8). Recent studies have shown that FoxM1 expression is negatively regulated by p53 (9,10). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (10,11). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (12).叉头框蛋白M1 (FoxM1)是一种叉头框家族的转录因子，调节细胞周期过程中的许多基因，有助于控制DNA复制、有丝分裂和细胞增殖。FoxM1在G1和S期表达增加，在G2/M期达到最大值(1-3)。核转移发生在G2/M期之前并和FoxM1的磷酸化有关(4)。FoxM1的磷酸化主要有MAPK (Ser331 Ser704)，Cyclin/Cdk (Thr600，Thr611，Thr672)，Plk1 (Ser715，Ser724)和Chk2 (Ser376)，可以稳定和激活FoxM1 (4-7)。FoxM1在所有的胚胎组织中表达，但是在成年人仅限于增生组织(8)。最近的研究表明p53可以负调控FoxM1的表达(9,10)。FoxM1的表达上调和许多人类肿瘤相关，包括前列腺癌，乳腺癌，肺癌，卵巢癌，结肠癌，胰腺癌，胃癌，膀胱癌，肝癌和肾癌，并可能与一些肿瘤中的p53突变有关(10,11)。因此，FoxM1抑制剂已成为潜在癌症治疗的研究热点(12)。 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or synchronized at the G1/S border by aphidicolin treatment (right), using FoxM1 (D12D5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测未处理的HeLa细胞（左图）或阿非迪霉素处理后同步化于G1/S期边缘的HeLa细胞（右图），使用的抗体为FoxM1 (D12D5) XP® Rabbit mAb ，为绿色。肌动蛋白纤维标记为DY-554鬼笔环肽，呈红色。 | |
Western blot analysis of extracts of HeLa cells, synchronized at the G1/S border by aphidicolin treatment (2 μM, 24 hours), using FoxM1 (D12D5) XP® Rabbit mAb. Western blot方法检测阿非迪霉素(2 μM, 24 小时)处理后同步化于G1/S期边缘的HeLa细胞提取物，使用的抗体为FoxM1 (D12D5) XP® Rabbit mAb。 |
