| 货号 | 5174S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | GAPDH (D16H11) XP® Rabbit mAb detects endogenous levels of total GAPDH protein. |
| 使用方法 | WB(1:1000) IHC-P (1:800) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimers diseases (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb. 免疫组化检测人乳腺癌石蜡切片，所用抗体为GAPDH (D16H11) XP® Rabbit mAb兔单抗 | |
Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb. Wetern blot检测多种细胞系提取物，所用抗体为GAPDH (D16H11) XP® Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测C2C12细胞提取物，所用抗体为GAPDH (D16H11) XP® Rabbit mAb兔单抗（绿色），微丝用DY-554 phalloidin染色(红色)。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) | |
Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb. |
