| 货号 | 4290S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | HER2/ErbB2 (D8F12) XP® Rabbit mAb detects endogenous levels of total HER/ErbB2 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:800) |
| 供应商 | CST |
| 背景 | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HER2/ErbB2 (D8F12) XP® Rabbit mAb.免疫组化检测石蜡包埋的人乳腺癌组织，使用抗体为：HER2/ErbB2 (D8F12) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded SK-BR-3 (Her2 high, left) and MCF-7 cell pellets (Her2 low, right) using HER2/ErbB2 (D8F12) XP® Rabbit mAb.免疫组化检测石蜡包埋的下列细胞沉淀：SK-BR-3 (Her2高表的,左)， MCF-7 (Her2低表达,右)。使用抗体为：HER2/ErbB2 (D8F12) XP® Rabbit mAb。 | |
Western blot analysis of extracts from SK-BR-3 and MCF-7 cells using HER2/ErbB2 (D8F12) XP® Rabbit mAb.Western blot法检测SK-BR-3 和MCF-7细胞提取物。使用抗体为：HER2/ErbB2 (D8F12) XP® Rabbit mAb。 |
