| 货号 | 7777S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | HMGA1 (D6A4) XP® Rabbit mAb recognizes endogenous levels of total HMGA1 protein, isoforms 1a and 1b. Based on sequence homology, this antibody is not predicted to cross-react with HMGA2. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:3200) |
| 供应商 | CST |
| 背景 | HMGA1, formerly known as HMG-I/Y, belongs to a family of high mobility group proteins that contain an AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA1 is highly expressed during embryogenesis and in embryonic stem cells, but not in fully differentiated adult tissues (2-4). Research studies have shown that HMGA1 is over-expressed in rapidly dividing neoplastic cells and a wide variety of aggressive cancers, including thyroid, colon, breast, pancreas, and prostate (2-4). Investigators have shown that forced expression of HMGA1 induces cellular transformation and an epithelial-to-mesenchymal transition (EMT), while inhibition of HMGA1 expression blocks anchorage-independent cell growth and proliferation of cancer cells, suggesting that HMGA1 contributes to carcinogenesis by inducing and maintaining a de-differentiated, highly proliferative cell state (5-8). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Confocal immunofluorescent analysis of NCCIT (high expression; left) or MCF7 (low expression; right) cells, using HMGA1 (D6A4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).采用共聚焦免疫荧光术检测NCCIT（高表达；左）和MCF7细胞（低表达；右），使用的抗体为HMGA1 (D6A4) XP® Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。蓝色假彩色= DRAQ5® #40 | |
Western blot analysis of extracts from various cell lines using HMGA1 (D6A4) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测不同细胞系的提取物，使用的抗体为HMGA1 (D6A4) XP® Rabbit mAb (上图) or β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Immunoprecipitation of HMGA1 from COS-7 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3) or HMGA1 (D6A4) XP® Rabbit mAb (lane 2). Lane 1 is 10% input. Western blot analysis was performed using HMGA1 (D6A4) XP® Rabbit mAb.从 COS-7细胞提取物中免疫沉淀HMGA1，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane3)或HMGA1 (D6A4) XP® Rabbit mAb (lane 2)。Lane 1为10% input。 使用HMGA1 (D6A4) XP® Rabbit mAb进行Western blot检测。 |
