货号 | 3890S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP/IHC-P/IF-IC/F |
目标/特异性 | HS1 (D83A8) XP® Rabbit mAb (Human Specific) detects endogenous levels of total HS1 protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels. |
使用方法 | WB(1:1000) IP (1:200) IHC-P (1:100) F (1:200) IF-IC (1:200) |
供应商 | CST |
背景 | HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7). |
存放说明 | -20C |
计算分子量 | 80 |
Western blot analysis of cell extracts from Raji, Jurkat, and Molt4 using HS1 (D83A8) XP® Rabbit mAb (Human Specific).Western免疫印迹。用HS1 (D83A8) XP® Rabbit mAb (Human Specific)研究Raji, Jurkat 和 Molt4 细胞的细胞提取液。 | |
Confocal immunofluorescent analysis of Jurkat cells (left) versus 293 cells (right) using HS1 (D83A8) XP® Rabbit mAb (Human Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析urkat细胞 (左图) 对比293细胞(右图),所用抗体为HS1 (D83A8) XP® Rabbit mAb (Human Specific)(绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Flow cytometric analysis of MCF-7 cells (red) and MOLT-4 cells (blue), using HS1 (D83A8) XP® Rabbit mAb (Human Specific).流式细胞仪分析MCF-7 细胞(红色)和MOLT-4 细胞 (蓝色),所用抗体为HS1 (D83A8) XP® Rabbit mAb (Human Specific)。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using HS1 (D83A8) XP® Rabbit mAb (Human Specific).免疫组织化学染色分析石蜡包埋人淋巴瘤组织。所用抗体为HS1 (D83A8) XP® Rabbit mAb (Human Specific)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HS1 (D83A8) XP® Rabbit mAb (Human Specific).免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为HS1 (D83A8) XP® Rabbit mAb (Human Specific)。 |