| 货号 | 8478S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | IRF-1 (D5E4) XP® Rabbit mAb recognizes endogenous levels of total IRF-1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). |
| 存放说明 | -20C |
| 计算分子量 | 45-48 |
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or treated with hIFNγ #8901 (100 ng/mL, 4 hr; right), using IRF-1 (D5E4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin.共聚焦免疫荧光分析HeLa细胞，血清饥饿(左图) 或hIFNγ #8901 (100 ng/mL, 4 hr)处理的(右图)，所用抗体为IRF-1 (D5E4) XP® Rabbit mAb(绿色)。肌动蛋白微丝用 DY-554 phalloidin 标记。 | |
Western blot analysis of HeLa cells, untreated or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 4 hr), using IRF-1 (D5E4) XP® Rabbit mAb.Western免疫印迹。用IRF-1 (D5E4) XP® Rabbit mAb研究未经处理的和经hIFN-γ #8901 (100 ng/ml, 4 hr)处理的HeLa细胞的细胞提取液。 | |
Western blot analysis of extracts from various cell lines using IRF-1 (D5E4) XP® Rabbit mAb.Western免疫印迹。用IRF-1 (D5E4) XP® Rabbit mAb 研究各种细胞的细胞提取液。 | |
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with Human Interferon-γ (hIFN-γ) #8901 (green), using IRF-1 (D5E4) XP® Rabbit mAb.流式细胞仪分析未经处理(蓝色)或经hIFN-γ #8901处理的(绿色)HeLa细胞，所用抗体为IRF-1 (D5E4) XP® Rabbit mAb。 | |
Imunohistochemical analysis of paraffin-embedded human breast carcioma using IRF-1 (D5E4) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为IRF-1 (D5E4) XP® Rabbit mAb，在对照多肽存在下(左图) 或者抗原特异性多肽(右图)。 | |
Imunohistochemical analysis of paraffin-embedded human lymphoma using IRF-1 (D5E4) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人淋巴瘤组织。所用抗体为IRF-1 (D5E4) XP® Rabbit mAb。 | |
Imunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or IFN gamma-treated (right), using IRF-1 (D5E4) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋HeLa细胞沉淀，对照(左图)或者 IFN gamma处理的(右图)。所用抗体为 IRF-1 (D5E4) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded mosue thymus using IRF-1 (D5E4) XP® Rabbit mAb. |
