| 货号 | 5519S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | JNK-Interacting Proteins (JIPs), as their name implies, coordinate c-Jun N-terminal Kinase (JNK) signaling by acting as scaffolds for components of the JNK signaling cascade (1). JIPs localize and promote JNK activation in response to stress by amalgamating and co-localizing upstream kinases and downstream effectors in the stress-kinase pathway analogous to the mechanism by which AKAPs orchestrate PKA signaling. JIPs bind to an array of MAPKs and other signaling proteins, including the mixed-lineage kinases, MKK7, p38α MAPK, JNK1-3, Max, Myc, NF-κB, LRRK2, and others (1-4). There are four known JIPs, JIP1-4, of which JIP1 and JIP2 share extensive sequence homology and domain structure. JIP1 and JIP2 are mainly expressed in neurons, testis and in β pancreatic cells, where they have been implicated in cellular responses to metabolic stress, the development of diabetes, and post-traumatic brain damage (5-7). Although architecturally distinct from JIP1 and JIP2, JIP3 and JIP4 share some overlapping functions and are more broadly expressed. JIP4, encoded by the SPAG9 (sperm-associated antigen-9) gene, is a homooligomer that binds to and coordinates the activation of numerous components of the stress-activated kinase cascade including MEK4, MEKK3, p38α MAPK, and JNK1-3 (3,8). However, unlike the other JIP members, JIP4 does not appear to activate JNK directly, instead favoring stimulation of p38 MAPK signaling events in response to cellular stress (3,9). In addition to mediating stress responses, JIP4 (or its splice variant, JLP) has also been shown to interact with ARF6 and PIKfyve, thus regulating microtubule-based endosomal trafficking (10,11). There are extensive reports indicating that JIP4 is phosphorylated in response to stress (UV damage etc.) but it is unclear what effect, if any, this has on its function, localization, or binding properties (12-15). |
| 存放说明 | -20C |
| 计算分子量 | 190 |
Confocal immunofluorescent analysis of HeLa cells untreated (left) or nocodazole-treated (right) using JIP4/SPAG9 (D72F4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测：未处理的（左侧）、诺考达唑处理的（右侧）HeLa 细胞，检测抗体为JIP4/SPAG9 (D72F4) XP® Rabbit mAb ，呈绿色。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 | |
Confocal immunofluorescent analysis of A172 cells using JIP4/SPAG9 (D72F4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测A172 细胞，检测抗体为JIP4/SPAG9 (D72F4) XP® Rabbit mAb，呈绿色。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 | |
Western blot analysis of extracts from various tissues and cell lines using JIP4/SPAG9 (D72F4) XP® Rabbit mAb.Western blot 方法检测细胞提取物：各种组织和细胞系，使用的抗体是JIP4/SPAG9 (D72F4) XP® Rabbit mAb。 | |
Flow cytometric analysis of NIH/3T3 cells using JIP4/SPAG9 (D72F4) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：NIH/3T3 细胞，使用的抗体是JIP4/SPAG9 (D72F4) XP® Rabbit mAb (蓝色)，红色表示非特异性阴性对照抗体。 | |
Immunohistochemical analysis of paraffin-embedded human lung adenocarcioma using JIP4/SPAG9 (D72F4) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcioma using JIP4/SPAG9 (D72F4) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). |
