| 货号 | 9091S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | LAMP1 (D2D11) XP® Rabbit mAb recognizes endogenous levels of total LAMP1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 42 (non-glycosylated), 90-120 (glycosylated) |
Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用LAMP1 (D2D11) XP® Rabbit mAb对HeLa细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.使用LAMP1 (D2D11) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of normal human kidney using LAMP1 (D2D11) XP(R) Rabbit mAb. | |
Immunohistochemical analysis of human lung carcinoma using LAMP1 (D2D11) XP(R) Rabbit mAb. | |
Immunohistochemical analysis of human ovarian serous cystadenoma using LAMP1 (D2D11) XP(R) Rabbit mAb. |
