| 货号 | 4599S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | LC3A (D50G8) XP® Rabbit mAb detects endogenous levels of total LC3A protein. This antibody may also react with LC3B. |
| 使用方法 | WB(1:1000) IHC-P (1:6400) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11). |
| 存放说明 | -20C |
| 计算分子量 | 14, 16 |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or chloroquine-treated (50 μM, overnight), using LC3A (D50G8) XP® Rabbit mAb. Western blot分析HeLa和NIH/3T3细胞的提取物，未处理或氯喹处理(50 μM, 过夜)，使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LC3A (D50G8) XP® Rabbit mAb (left) orRabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). | |
免疫组化分析石蜡包埋的人结肠癌，使用LC3A (D50G8) XP® Rabbit mAb 兔单抗（左边）或 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（右边）。 | |
Immunohistochemical analysis of paraffin-embedded human glioblastoma multiforme using LC3A (D50G8) XP® Rabbit mAb. 免疫组化分析石蜡包埋的人多形性胶质母细胞瘤，使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of Chloroquine-treated HeLa cells, using LC3A (D50G8) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 流式细胞术分析氯喹处理的HeLa细胞，使用的抗体是LC3A (D50G8) XP® Rabbit mAb 兔单抗（蓝色）Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（红色）作为对照。 |
