| 货号 | 12741S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | LC3A/B (D3U4C) XP® Rabbit mAb recognizes endogenous levels of total LC3A and LC3B proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:1000) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). |
| 存放说明 | -20C |
| 计算分子量 | 14, 16 |
Confocal immunofluorescent analysis of HeLa (upper) and C2C12 (lower) cells, chloroquine-treated (50 μM, overnight; left), nutrient-starved with EBSS (3 hr, middle) or untreated (right) using LC3A/B (D3U4C) XP® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa（上图）和C2C12（下图）细胞，氯喹处理(50 μM, 过夜; 左)， EBSS营养饥饿处理(3 小时, 中)或未处理（右），使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗（绿色）和 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) 兔单抗#8046（红色）。蓝色伪彩= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from RD cells, untreated (-) or Torin 1-treated (250 nM, 4 hr; +), using LC3A/B (D3U4C) XP® Rabbit mAb. Western blot分析RD细胞的提取物，未处理(-)或 Torin 1 (托林1)处理(250 nM, 4小时; +)，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from HeLa, NIH/3T3, and KNRK cells, untreated (-) or chloroquine-treated (50 μM, overnight; +), using LC3A/B (D3U4C) XP® Rabbit mAb. Western blot分析HeLa，NIH/3T3和KNRK细胞的提取物，未处理(-)或氯喹处理(50 μM, 过夜; +)，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellets, control (left) or chloroquine-treated (right), using LC3A/B (D3U4C) XP® Rabbit mAb. 免疫组化分析石蜡包埋的NIH/3T3细胞沉淀，对照（左）或氯喹处理（右），使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LC3A/B (D3U4C) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化分析石蜡包埋的人肺癌组织，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗，分别经对照肽（左），或抗原特异性多肽（右）处理。 | |
Immunohistochemical analysis of paraffin-embedded mouse prostate using LC3A/B (D3U4C) XP® Rabbit mAb. 免疫组化分析石蜡包埋的小鼠前列腺，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with chloroquine (50 µM, 16 hr) (green), using LC3A/B (D3U4C) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. |
