| 货号 | 5471S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Livin (D61D1) XP® Rabbit mAb detects endogenous levels of total livin protein. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). |
| 存放说明 | -20C |
| 计算分子量 | 34, 36 |
Western blot analysis of extracts from various cell lines using Livin (D61D1) XP® Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为 Livin (D61D1) XP® Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of SK-MEL-28 cells (left) and HeLa cells (right) using Livin (D61D1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 共聚焦免疫荧光分析SK-MEL-28细胞（左）和HeLa细胞（右），使用抗体为Livin (D61D1) XP® Rabbit mAb 兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。 |
