| 货号 | 8707S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | MAP2 (D5G1) XP® Rabbit mAb recognizes endogenous levels of total MAP2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) |
| 供应商 | CST |
| 背景 | Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3). |
| 存放说明 | -20C |
| 计算分子量 | 75, 82, 280 |
Confocal immunofluorescent analysis of frozen rat cerebellum using MAP2 (D5G1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析冷冻大鼠小脑，使用的抗体是MAP2 (D5G1) XP® Rabbit mAb兔单抗（绿色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from neonatal and adult mouse brain using MAP2 (D5G1) XP® Rabbit mAb. Western blot 检测新生和成年小鼠大脑提取物，使用的抗体是 MAP2 (D5G1) XP® Rabbit mAb兔单抗。 |
