| 货号 | 5607S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | MARCKS (D88D11) XP® Rabbit mAb recognizes endogenous levels of total MARCKS protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Flow cytometric analysis of Jurkat (blue) and HeLa (green) cells using MARCKS (D88D11) XP® Rabbit mAb. 对Jurkat（蓝）和Hela（绿）细胞使用MARCKS (D88D11) XP®兔单抗进行共聚焦免疫荧光分析。 | |
Confocal immunofluorescent analysis of HeLa (left), MCF-7 (middle), and Jurkat (right) cells using MARCKS (D88D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
对HeLa（左）、MCF-7（中）和Jurkat（右）细胞使用MARCKS (D88D11) XP®兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of cell extracts from various cell lines using MARCKS (D88D11) XP® Rabbit mAb. 对多种细胞系使用MARCKS (D88D11) X®兔单克隆抗体进行Western blot分析。 | |
Immunohistochemical analys of paraffin-embedded human breast carcinoma using MARCKS (D88D11) XP® Rabbit mAb. 对石蜡包埋的人乳腺使用MARCKS (D88D11) XP®兔单抗进行免疫组化分析。 |
