| 货号 | 3456S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | MeCP2 (D4F3) XP® Rabbit mAb detects endogenous levels of MeCP2 (both isoforms A and B). This antibody does not cross-react with other MBD proteins. |
| 使用方法 | WB(1:1000) IP (1:25) IHC-P (1:1600) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Methyl-CpG-binding protein 2 (MeCP2) is the founding member of a family of methyl-CpG-binding domain (MBD) proteins that also includes MBD1, MBD2, MBD3, MBD4, MBD5 and MBD6 (1-3). Apart from MBD3, these proteins bind methylated cytosine residues in the context of the di-nucleotide 5´-CG-3´ to establish and maintain regions of transcriptionally inactive chromatin by recruiting a variety of co-repressor proteins (2). MeCP2 recruits histone deacetylases HDAC1 and HDAC2, and the DNA methyltransferase DNMT1 (4-6). MBD1 couples transcriptional silencing to DNA replication and interacts with the histone methyltransferases ESET and SUV39H1 (7,8). MBD2 and MBD3 co-purify as part of the NuRD (nucleosome remodeling and histone de-acetylation) co-repressor complex, which contains the chromatin remodeling ATPase Mi-2, HDAC1 and HDAC2 (9,10). MBD5 and MBD6 have recently been identified and little is known regarding their protein interactions. MBD proteins are associated with cancer and other diseases; MBD4 is best characterized for its role in DNA repair and MBD2 has been linked to intestinal cancer (11,12). Mutations in the MeCP2 gene cause the neurologic developmental disorder Rett Syndrome (13). MeCP2 protein levels are high in neurons, where it plays a critical role in multiple synaptic processes (14). In response to various physiological stimuli, MeCP2 is phosphorylated on Ser421 and regulates the expression of genes controlling dendritic patterning and spine morphogenesis (14). Disruption of this process in individuals with altered MeCP2 may cause the pathological changes seen in Rett Syndrome. |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Confocal immunofluorescent analysis of SH-SY5Y cells using MeCP2 (D4F3) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用MeCP2 (D4F3) XP® Rabbit mAb (绿色)，共聚焦免疫荧光分析SH-SY5Y细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from various cell lines using MeCP2 (D4F3) XP® Rabbit mAb. 使用MeCP2 (D4F3) XP® Rabbit mAb，免疫印迹(Western blot)分析不同细胞中MeCP2 (D4F3)的蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MeCP2 (D4F3) XP® Rabbit mAb. 使用MeCP2 (D4F3) XP® Rabbit mAb，免疫组化分析人源肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain using MeCP2 (D4F3) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right). 使用MeCP2 (D4F3) XP® Rabbit mAb，control peptide (左图)和antigen-specific peptide (右图)，免疫组化分析小鼠大脑组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using MeCP2 (D4F3) XP® Rabbit mAb. |
