| 货号 | 4319S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Mer (D21F11) XP® Rabbit mAb detects endogenous levels of total Mer protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Mer tyrosine kinase belongs to a receptor tyrosine kinase family with Axl and Tyro-3. This family is characterized by a common N-CAM (neural adhesion molecule)-related extracellular domain and a common ligand, GAS6 (growth arrest-specific protein 6). Mer protein has an apparent molecular weight of 170-210 kDa due to different glycosylation pattern generated in different cell types. Mer can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif (1). The downstream signaling components of activated Mer include PI3 kinase, PLCγ and MAP kinase (2). Family members are prone to transcriptional regulation and carry out diverse functions including the regulation of cell adhesion, migration, phagocytosis, and survival (3). Mer regulates macrophage activation, promotes apoptotic cell engulfment, and supports platelet aggregation and clot stability in vivo (4). Overexpression of Mer may play a cooperative role in leukemogenesis and may be an effective target for biologically based leukemia/lymphoma therapy (5). |
| 存放说明 | -20C |
| 计算分子量 | 210 |
Western blot analysis of extracts from SK-MEL-5, Hep G2 and Jurkat cells using Mer (D21F11) XP® Rabbit mAb.用抗体Mer (D21F11) XP® Rabbit mAb对SK-MEL-5、 Hep G2和 Jurkat细胞的提取物进行免疫印迹检测。 | |
Confocal immunofluorescent analysis of SK-MEL-5 cells (left), Hep G2 cells (center) and MCF7 cells (right) using Mer (D21F11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测SK-MEL-5 (左)、 Hep G2（中）和MCF7（右）细胞，使用抗体为：Mer (D21F11) XP® Rabbit mAb（绿色）。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 |
