| 货号 | 8198S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Met (D1C2) XP® Rabbit mAb recognizes endogenous levels of total Met protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:300) F (1:400) IF-IC (1:3000) IHC-F (1:200) |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 140, 170 |
Western blot analysis of extracts from HT-29 (Met+), SK-BR-3 (Met-), and T-47D (Met-) cells using Met (D1C2) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).用Met (D1C2) XP® Rabbit mAb对HT-29 (Met+), SK-BR-3 (Met-), 和 T-47D (Met-)细胞的提取物进行免疫印迹检测。 | |
Immunohistochemical analysis of frozen MKN-45 xenograft using Met (D1C2) XP® Rabbit mAb.用Met (D1C2) XP® Rabbit mAb对MKN-45细胞异种移植物的冰冻组织进行免疫印迹检测。 | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Met (D1C2) XP® Rabbit mAb.用Met (D1C2) XP® Rabbit mAb对石蜡包埋的人肝癌组织进行免疫印迹检测。 | |
Immunohistochemical analysis of paraffin-embedded human metastatic lung carcinoma using Met (D1C2) XP® Rabbit mAb.用Met (D1C2) XP® Rabbit mAb对石蜡包埋的人转移性肺癌组织进行免疫印迹检测。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, MKN-45 (left) and T-47D (right), using Met (D1C2) XP® Rabbit mAb.用Met (D1C2) XP® Rabbit mAb对石蜡包埋的MKN-45 (左)和T-47D（右）细胞的团块进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Met (D1C2) XP® Rabbit mAb.用Met (D1C2) XP® Rabbit mAb对石蜡包埋的乳头状肾细胞癌组织进行免疫印迹检测。 | |
Confocal immunofluorescent analysis of HT-29 and T-47D cells using Met (D1C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测HT-29 和 T-47D细胞，使用抗体为：Met (D1C2) XP® Rabbit mAb（绿色）。肌动蛋白微丝用DY-554 phalloidin (红色)标记。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 | |
Flow cytometric analysis of T-47D cells (blue) and HT-29 cells (green) using Met (D1C2) XP® Rabbit mAb.用Met (D1C2) XP® Rabbit mAb对 T-47D细胞 (蓝色)和HT-29细胞 (绿色)进行流式细胞术检测。 |
