| 货号 | 6915S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | Miwi (D92B7) XP® Rabbit mAb recognizes endogenous levels of total Miwi protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:800) |
| 供应商 | CST |
| 背景 | The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Confocal immunofluorescent analysis of mouse testis using Miwi (D92B7) XP® Rabbit mAb. Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Miwi (D92B7) XP® Rabbit mAb兔单抗（绿色），共聚焦免疫荧光观察Miwi蛋白在小鼠睾丸组织中定位。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Western blot analysis of extracts from mouse testis and mouse brain using Miwi (D92B7) XP® Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). 使用Miwi (D92B7) XP® Rabbit mAb兔单抗 (上图)和β-Actin (13E5) Rabbit mAb兔单抗 #4970 (下图)，免疫印迹（Western blot）分析小鼠睾丸和大脑组织的裂解物。 |
