| 货号 | 3679S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Mitogen- and stress-activated protein kinase 1 (MSK1) and MSK2 are serine/threonine kinases that promote immediate early gene transcription in stress- or mitogen-induced cells (1-4,7, 8) and LPS-stimulated macrophages (9). MSK2, also known as RSKB, contains two catalytic domains and has been shown to interact directly with p38 MAP kinase (10). MSK2 is phosphorylated and activated in response to tumor necrosis factor, epidermal growth factor or phorbol ester in HeLa cells or murine embryonic fibroblasts (MEFs) in a p38- and ERK-dependent manner (8,11). Phosphorylation on residues Ser196 and Thr568 within the activation loop of both catalytic domains is required for full kinase activation (11). Both MSK1 and MSK2 contain a functional nuclear localization sequence that is sufficient and required for nuclear targeting (10). Consistent with their nuclear localization, these kinases play an important role in regulating transcriptional responses to stress and mitogens. Activation of MSK2 in HeLa cells or MEFs results in rapid phosphorylation of histone H3, HMG-14, CREB and ATF1 and acetylation of histone H3 associated with immediate early gene transcription (3,4,6,7). |
| 存放说明 | -20C |
| 计算分子量 | 85, 90 |
Western blot analysis of extracts from HeLa and A-431 cells, untreated or treated with Calyculin A #9902 (100 nM, 30 minutes), using MSK2 (D41A4) XP® Rabbit mAb.Western blot方法检测细胞提取物：未处理、calyculin A#9902 (100 nM, 30 min)处理的HeLa、 A-431细胞。使用的抗体是MSK2 (D41A4) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of A-431 cells using MSK2 (D41A4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测A-431细胞，使用的抗体是MSK2 (D41A4) XP® Rabbit mAb (绿色)。肌动蛋白丝被 DY-554鬼笔环肽标记，呈红色。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084）。 |
