| 货号 | 9485S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | NDRG1 (D8G9) XP® Rabbit mAb recognizes endogenous levels of total NDRG1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:800) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3. |
| 存放说明 | -20C |
| 计算分子量 | 46, 48 |
Confocal immunofluorescent analysis of 786-0 cells (left) and A-204 cells (right) using NDRG1 (D8G9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用NDRG1 (D8G9) XP® Rabbit mAb (绿色)对786-0 cells (左) 和 A-204 细胞(右)进行共聚焦免疫荧光分析，肌蛋白丝标记DY-554 phalloidin (红色)，蓝色伪彩= DRAQ5® #4084 (DNA荧光染料). | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using NDRG1 (D8G9) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 在对照肽段或抗原特异肽段存在的情况下，使用DRG1 (D8G9) XP® Rabbit mAb兔单抗对石蜡包埋的人淋巴癌组织进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using NDRG1 (D8G9) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).. 使用 NDRG1 (D8G9) XP® Rabbit mAb (上) 和 β-Actin (D6A8) Rabbit mAb #8457 （下）等抗体对多种细胞提取物进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using NDRG1 (D8G9) XP® Rabbit mAb. | |
使用DRG1 (D8G9) XP® Rabbit mAb兔单抗对石蜡包埋的人结肠癌组织进行免疫组化分析。 |
