| 货号 | 5861S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | NFAT1 (D43B1) XP® Rabbit mAb detects endogenous levels of total NFAT1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:50) F (1:800) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response (1,2). NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells (3). In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus (2). Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1 (3,4). Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm (5). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Ionomycin #9995 (1 μM, 1 hr; right), using NFAT1 (D43B1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析未经处理的 (左图)或经Ionomycin #9995 (1 μM, 1 hr; 右图)处理的MCF7细胞，所用抗体为NFAT1 (D43B1) XP® Rabbit mAb (绿色)。肌动蛋白丝用 DY-554 phalloidin 标记(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Flow cytometric analysis of HCT 116 using NFAT1 (D43B1) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).流式细胞仪研究HCT 116 细胞，所用抗体为NFAT1 (D43B1) XP® Rabbit mAb (蓝色)与浓度相匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)对比。 | |
Western blot analysis of extracts from Ramos, Raji, and EL4 cells using NFAT1 (D43B1) XP® Rabbit mAb.Western免疫印迹。用NFAT1 (D43B1) XP® Rabbit mAb研究 Ramos, Raji 和 EL4 细胞的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, Jurkat (left) or LNCaP (right), using NFAT1 (D43B1) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using NFAT1 (D43B1) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using NFAT1 (D43B1) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using NFAT1 (D43B1) XP® Rabbit mAb. |
