NF-κB p65 (D14E12) XP® Rabbit mAb【科瑞奥博】

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NF-κB p65 (D14E12) XP® Rabbit mAb

货号： 8242S 基本售价： 3860.0 元规格： -

产品信息

概述
货号	8242S
反应种属	Human/Mouse/Rat/Hamster/MonkeyDog
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-IC/F/ChIP
目标/特异性	NF-κB p65 (D14E12) XP® Rabbit mAb recognizes endogenous levels of total NF-κB p65/RelA protein. It does not cross react with other NF-κB/Rel family members.
使用方法	WB(1:1000) IP (1:100) IHC-P (1:800) F (1:1600) IF-IC (1:400) ChIP (1:100)

性能
供应商	CST
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
存放说明	-20C
计算分子量	65

参考图片

Confocal immunofluorescent analysis of HT-1080 cells, untreated (left) or treated with hTNF-α #8902 (20 ng/ml, 20 min) (right), using NF-κB p65 (D14E12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HT-1080 细胞核，未经处理 (左图)或经hTNF-α #8902 (20 ng/ml, 20 min) (右图)处理, 所用抗体为NF-κB p65 (D14E12) XP® Rabbit mAb (绿色) 肌动蛋白微丝用DY-554 phalloidin标记（红色)。Blue pseudocolor=DRAQ5® #4084 DNA 荧光染料。

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with hTNF-α #8902 (30 ng/ml, 1 hr) and either 5 μl of NF-κB p65 (D14E12) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, human IL-8 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。HeLa细胞培养至4 x 106，并用hTNF-α #8902 (30 ng/ml, 1 hr)处理后，将染色质交联到玻片上,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用5 μl of NF-κB p65 (D14E12) XP® Rabbit mAb抗体或2 μl Normal Rabbit IgG #2729 。对富集的DNA做real-time PCR,所用引物为SimpleChIP® Human IκBα Promoter Primers #5552, human IL-8 promoter primers和SimpleChIP® Human α Satellite Repeat Primers #4486。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Western blot analysis of extracts from various cell lines using NF-κB p65 (D14E12) XP® Rabbit mAb.Western免疫印迹。用NF-κB p65 (D14E12) XP® Rabbit mAb研究各种类型细胞的细胞提取液。

Flow cytometric analysis of HeLa cells using NF-κB p65 (D14E12) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).流式细胞仪研究HeLa细胞，所用抗体为NF-κB p65 (D14E12) XP® Rabbit mAb (蓝色) 浓度相匹配的 Rabbit (DA1E) mAb IgG XP® 同型对照#3900 (红色)。

Immunohistochemical analysis of paraffin-embedded human chronic cholecystitis using NF-κB p65 (D14E12) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人慢性胆囊炎组织。所用抗体为NF-κB p65 (D14E12) XP® Rabbit mAb。