| 货号 | 3924S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | NSF (D31C7) XP® Rabbit mAb detects endogenous levels of total NSF protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-F (1:100) |
| 供应商 | CST |
| 背景 | Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from various cell types using NSF (D31C7) XP® Rabbit mAb. 对多种细胞系使用NSF (D31C7) XP® Rabbit mAb兔单抗进行Western blot分析。 | |
Confocal immunofluorescent analysis of mouse brain using NSF (D31C7) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对小鼠脑使用NSF (D31C7) XP® Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 |
