| 货号 | 3669S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). G-protein coupled receptors (GPCRs) at the cell surface signal through heteromeric G proteins to small GTPases such as Rho, which then signal to downstream effector molecules (2). p115 RhoGEF/ArhGEF1 and its family members PDZ-RhoGEF (PRG), and LARG are stimulated by heteromeric G proteins and thus couple signaling from GPCRs to Rho small GTPases (3-6). In a mouse model of asthma, p115 RhoGEF is necessary for T cells to enable airway inflammation and hyperreactivity (7).Rho family small GTPases包括Rho、Rac和cdc42，该家族作为分子开关，调节细胞进程例如细胞迁移、粘附、扩增和分化。它们通过鸟苷酸交换因子（guanine nucleotide exchange factor，GEF)被激活，这将催化已结合的GDP交换成GTP，然而通过GTPase activating proteins (GAPs)被抑制，这将催化GTP的水解成GDP。第三个调节水平通过Rho GDP dissociation inhibitor (RhoGDI)的计量结合力被提供(1)。在细胞表面G-protein coupled receptors (GPCRs)通过异质G 蛋白到small GTPases例如Rho从而发出信号，然而信号传递到下游效应分子 (2)。p115 RhoGEF/ArhGEF1和它的家族成员PDZ-RhoGEF (PRG)以及LARG通过异质G 蛋白被激活，因此从GPCRs到Rho small GTPases 的信号是成对(3-6)。在小鼠哮喘模型中，p115 RhoGEF蛋白对于T细胞进入气道炎症和其反应性是必须的 (7)。 |
| 存放说明 | -20C |
| 计算分子量 | 115 |
Western blot analysis of extracts from various cell types using p115 RhoGEF (D25D2) XP® Rabbit mAb.使用p115 RhoGEF (D25D2) XP® Rabbit mAb，免疫印迹（Western Blot）分析不同细胞系p115 RhoGEF (D25D2)蛋白水平。 | |
Immunoprecipitation of p115 RhoGEF from COS cells using p115 RhoGEF (D25D2) XP® Rabbit mAb. Western blot was preformed using the same antibody. Lane 1 is 5% input.使用p115 RhoGEF (D25D2) XP® Rabbit mAb，免疫沉淀分析COS细胞系中p115 RhoGEF蛋白。使用同样的抗体进行免疫印迹实验。第1道是5% input。 | |
Confocal immunofluorescent analysis of HEK/293 cells using p115 RhoGEF (D25D2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).使用p115 RhoGEF (D25D2) XP® Rabbit mAb（绿色），共聚焦免疫荧光分析HEK/293细胞系。蓝色= DRAQ5®（DNA荧光染料）。 |
