| 货号 | 8238S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | p75NTR (D4B3) XP® Rabbit mAb recognizes endogenous levels of total p75NTR protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | The p75 neurotrophin receptor (p75NTR), a member of the TNF receptor superfamily, is distinguished by multiple cysteine-rich ligand-binding domains, a single transmembrane sequence and a noncatalytic cytoplasmic domain (1). p75NTR displays paradoxical functions when acting alone or with other receptor proteins. Working in concert with Trk receptors, p75NTR recognizes neurotrophins and transmits trophic signals into the cell. Both p75NTR and TrkA are required to activate PI3K-Akt signaling, while TrkA can individually activate the MAP kinase pathway. In contrast, p75NTR, possibly through JNK, ensures appropriate apoptosis of injured neurons and improperly targeted neonatal neurons (2). The p75NTR protein undergoes sequential cleavage similar to APP and Notch. First, α-secretase removes the p75NTR ectodomain, eliminating ligand-mediated signaling. At this point, the membrane-tethered cleavage product can still fine-tune Trk-mediated trophic actions. γ-secretase cleaves within the transmembrane domain to liberate the cytoplasmic tail from its membrane anchor and allow the p75NTR intracellular domain to translocate to the nucleus (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Confocal immunofluorescent analysis of SK-N-MC cells using p75NTR (D4B3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析SK-N-MC细胞，使用p75NTR (D4B3) XP? Rabbit mAb 兔单抗（绿色）进行标记。肌动蛋白纤丝用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5 ® #4084(荧光DNA染料)。 | |
Western blot analysis of extracts from PC-3 cells, untreated (-) or ibuprofen-treated (2 mM, 24 hr) (+), and SW480 cells (-) using p75NTR (D4B3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). Western blot分析PC-3细胞，未处理(-)或2mM ibuprofen处理24小时(+)，和SW480细胞(-)提取物，使用的抗体是Pp75NTR (D4B3) XP® Rabbit mAb 兔单抗（上图）或Akt (pan) (C67E7) Rabbit mAb 兔单抗#4691（下图）。 |
