| 货号 | 12709S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | PAX5 (D7H5X) XP® Rabbit mAb recognizes endogenous levels of total PAX5 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintains functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Confocal immunofluorescent analysis of Ramos (left) and Jurkat (right) cells using PAX5 (D7H5X) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
使用PAX5 (D7H5X) XP® Rabbit mAb（绿色）对Ramos（左）和Jurkat（右）细胞进行共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin标记（红色）。蓝色伪彩= DRAQ5® #4084 (DNA荧光染色). | |
Western blot analysis of extracts from various cell lines using PAX5 (D7H5X) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). 使用PAX5 (D7H5X) XP® 兔mAb（上）和β-actin (D6A8)Rabbit mAb #8457对多种细胞提取物进行western blot分析。 |
