| 货号 | 9535S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | PDCD4 (D29C6) XP® Rabbit mAb detects endogenous levels of total PDCD4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:600) |
| 供应商 | CST |
| 背景 | PDCD4 (programmed cell death protein 4) was identified as a suppressor of neoplastic transformation (1). It interacts with eukaryotic initiation factors eIF4AI and eIF4AII (2). PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase (2). PDCD4 inhibits cap-dependent translation (2). Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth (3). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Immunohistochemical analysis of paraffin-embedded human tonsil using PDCD4 (D29C6) XP® Rabbit mAb. 采用PDCD4 (D29C6) XP® Rabbit mAb 兔单抗对石蜡包埋的的人扁桃体进行免疫组化分析。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or serum-starved (right), using PDCD4 (D29C6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 共聚焦免疫荧光分析未处理（左图）或血清饥饿的（右图）Hela细胞。肌动蛋白用DY554 phalloidin 标记（红色）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PDCD4 (D29C6) XP® Rabbit mAb. 采用PDCD4 (D29C6) XP® Rabbit mAb 兔单抗对石蜡包埋的人乳腺癌进行免疫组织化学分析（IHC）。 | |
Western blot analysis of extracts from various cell types using PDCD4 (D29C6) XP® Rabbit mAb. 采用 PDCD4 (D29C6) XP® Rabbit mAb 兔单抗对不同类型的细胞进行Western Blot分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse forestomach using PDCD4 (D29C6) XP® Rabbit mAb. |
