| 货号 | 9349S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F/IF-IC |
| 目标/特异性 | Perilipin (D1D8) XP® Rabbit mAb detects endogenous levels of total perilipin protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) IF-F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
Confocal immunofluorescent analysis of 8-day differentiated 3T3-L1 cells, using Perilipin (D1D8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 免疫荧光共聚焦显微镜观察分化8天的3T3-L1细胞，所用抗体为Perilipin (D1D8) Rabbit mAb兔单抗(绿色)。蓝色伪彩为 DRAQ5® #4084(DNA荧光染料)。 | |
Confocal immunofluorescent analysis of frozen mouse brown adipose tissue using Perilipin (D1D8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 免疫荧光共聚焦显微镜观察小鼠棕色脂肪冰冻组织，所用抗体为Perilipin (D1D8) XP® Rabbit mAb兔单抗 (绿色)。蓝色伪彩为DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from human pre-adipocytes and adipocytes using Perilipin (D1D8) XP® Rabbit mAb (upper) and β-Actin Antibody #4967 (lower). Western blot检测人前脂肪细胞和脂肪细胞提取物，所用抗体为Perilipin (D1D8) XP® Rabbit mAb (上)和β-Actin Antibody #4967(下)。 | |
Immunohistochemical analysis of paraffin-embedded breast carcinoma using Perilipin (D1D8) XP® Rabbit mAb. Note specific staining of adipocytes. 免疫组化检测乳腺癌组织石蜡切片，所用抗体为Perilipin (D1D8) XP® Rabbit mAb兔单抗。注意图中脂肪细胞的特异性染色。 | |
Immunohistochemical analysis of parafin-embedded mouse brown fat using Perilipin (D1D8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化检测小鼠棕色脂肪石蜡切片，左图为对照肽段，右图为抗原特异性肽段。所用抗体为Perilipin (D1D8) XP® Rabbit mAb兔单抗。 |
