| 货号 | 70076S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | WB,IP,IHC |
| 目标/特异性 | F4/80 (D2S9R) XP® Rabbit mAb recognizes endogenous levels of total F4/80 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) Immunohistochemistry (Paraffin) (1:250) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). |
| 存放说明 | -20C |
| 计算分子量 | 65-250 |
| 参考文献 | 1 . Hirsch, S. et al. (1981) J Exp Med 154, 713-25. 2 . Austyn, J.M. and Gordon, S. (1981) Eur J Immunol 11, 805-15. 3 . McKnight, A.J. et al. (1996) J Biol Chem 271, 486-9. |
Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or Neuro-2A cell pellet (right, negative) using F4/80 (D2S9R) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using F4/80 (D2S9R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded mouse liver using F4/80 (D2S9R) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using F4/80 (D2S9R) XP® Rabbit mAb. | |
Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using F4/80 (D2S9R) XP® rabbit mAb (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD3ε (D4V8L) rabbit mAb #99940 (red), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan). |
