| 货号 | 7067S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-F/IF-IC |
| 使用方法 | WB(1:1000) IHC-P (1:600) IF-F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2) is an alternatively spliced variant of M1 that is expressed during embryonic development (1). Research studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect (1). When cancer cells switch from the M2 isoform to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies showed that PKM2 is not essential for all tumor cells (4). In the tumor model studied, PKM2 was found to be active in the non-proliferative tumor cell population and inactive in the proliferative tumor cell population (4). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Confocal immunofluorescent analysis of COS-7 cells, transiently transfected with PKM1 (left) or PKM2 (right), using PKM1 (D30G6) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
使用PKM1 (D30G6) XP®Rabbit mAb兔单抗 (绿色)对瞬转了PKM1(左)或PKM2(右)的COS-7细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DyLight™ 554 Phalloidin #13054 (红色)标记。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Confocal immunofluorescent analysis of mouse skeletal muscle (left) or liver (right) using PKM1 (D30G6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye). 使用PKM1 (D30G6) XP® Rabbit mAb兔单抗(绿色)对小鼠骨骼肌(左)或肝脏(右)进行激光共聚焦荧光分析。蓝色伪彩= RAQ5® #4084 (fluorescent DNA dye)。 | |
Immunohistochemical analysis of paraffin-embedded human colon using PKM1 (D30G6) XP®Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 使用PKM1 (D30G6) XP® Rabbit mAb兔单抗在对照肽(左)或抗原特异性肽段(右)存在的条件下对石蜡包埋的人结肠组织进行免疫组化分析。 |
