| 货号 | 4053S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | PKM2 (D78A4) XP® Rabbit mAb detects endogenous levels of total PKM2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4). A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from various cell lines and mouse skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb (upper) or GAPDH (14C10) Rabbit mAb #2118. Wetern blot检测多种细胞和小鼠骨骼肌提取物，所用抗体为PKM2 (D78A4) XP＠ Rabbit mAb兔单抗 (上)和 GAPDH (14C10) Rabbit mAb兔单抗 #2118(下)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PKM2 (D78A4) XP® Rabbit mAb. 免疫组织化学检测石蜡包埋的人肺癌组织，所用抗体为PKM2 (D78A4) XP＠ Rabbit mAb兔单抗 | |
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb. Note the lack of staining in the skeletal muscle cells which do not express PKM2 while vessels within the tissue stain positively. 免疫组织化学检测人石蜡包埋的骨骼肌组织，所用抗体为PKM2 (D78A4) XP＠ Rabbit mAb兔单抗。留意有未表达PKM2的骨骼肌细胞中未着色，而组织中的血管阳性染色 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using PKM2 (D78A4) XP® Rabbit mAb. 免疫组织化学检测石蜡包埋的人淋巴瘤组织，所用抗体为PKM2 (D78A4) XP＠ Rabbit mAb兔单抗 | |
Confocal immunofluorescent analysis of A204 cells using PKM2 (D78A4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测A204 细胞，所用抗体为PKM2 (D78A4) XP＠ Rabbit mAb兔单抗（绿），蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084). |
