| 货号 | 5690S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | PLCγ1 (D9H10) XP® Rabbit mAb recognizes endogenous levels of total PLCγ1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1245 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
Immunohistochemical analysis of paraffin-embedded human lung carcioma using PLCγ1 (D9H10) XP® Rabbit mAb. 对石蜡包埋的肺癌使用PLCγ1 (D9H10) XP® 兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PLCγ1 (D9H10) XP® Rabbit mAb. 对石蜡包埋的人乳腺癌使用PLCγ1 (D9H10) XP® 兔单抗进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using PLCγ1 (D9H10) XP® Rabbit mAb. 对多种细胞系使用PLCγ1 (D9H10) XP®兔单克隆抗体进行Western blot分析。 | |
Western blot analysis of extracts from HeLa cells, either mock-transfected or transfected for 48 hours with SignalSilence™ PLCγ1 siRNA I #6293 or siRNA II #6254, using PLCγ1 (D9H10) XP® Rabbit mAb. 对HeLa细胞，mock阴性对照转染或转染SignalSilence PLCγ1 siRNA II #625448小时，使用PLCγ1(D9H10) XP®兔单克隆抗体进行Western blot分析。 |
