| 货号 | 74775S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | W/ChIP |
| 使用方法 | WB(1:1000) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the AAA+ (ATPase associated with diverse cellular activities) superfamily of proteins, and are putatively homologous to bacterial RuvB proteins that drive branch migration of Holliday junctions (1). Reptin and Pontin function together as essential components of chromatin remodeling and modification complexes, such as INO80, TIP60, SRCAP, and Uri1, which play key roles in regulating gene transcription (1,2). In their capacity as essential transcriptional co-regulators, Reptin and Pontin have both been implicated in oncogenic transformations, including those driven by c-Myc, β-catenin, and E1A (2-7). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Western blot analysis of extracts from various cell lines using Pontin/RUVBL1 (D1L8J) XP® Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT-15 cells treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml; 45 min) and either 10 μl of Pontin/RUVBL1 (D1L8J) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP®Human C/EBP-δ Primers #48781 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
