| 货号 | 3549S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The α isoform of protein phosphatase 2C (PP2C-α) is the catalytic subunit of a widely expressed serine/threonine phosphatase involved in regulation of the cell stress response (1,2). Also known as magnesium-dependent protein phosphatase (PPM1A), this monomeric phosphatase is a member of a conserved group of proteins that acts on many different substrates in numerous pathways. PP2C-α inhibits p38 MAPK and SAPK/JNK pathways activated in response to cell stress as seen in both in vivo and in vitro studies. Specifically, PP2C-α removes phosphates from MKK3 and MKK7, reducing activity of both proteins and inhibiting activation of the downstream kinases JNK and p38 MAPK, respectively (3). Another PP2C-α substrate is IKKβ, the critical regulator of NF-κB signaling. Dephosphorylation of IKKβ at Ser177/181 by PPM1A and PPM1B results in inactivation of IKKβ and inhibition of NF-κB signaling (4). PP2C-α is one of the phosphatases responsible for removing phosphate residues from cyclin dependent protein kinases. In a study using HeLa cell extracts, PP2C-α dephospohrylates CDK2 and CDK6, with a preference toward interacting with CDK2 phosphorylated at Thr160, a residue found in the activating T-loop of the kinase. Removal of phosphates from this site is thought to inactivate cyclin-associated kinases (5). PP2C-α induces cell cycle arrest and apoptosis, likely through activation of p53 though other pathways may also contribute to PP2C-α mediated cell death (6). Additional PP2C-α substrates include the Wnt signaling pathway protein axin (7) and CFTR, a chloride channel protein implicated in cystic fibrosis (8). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Flow cytometric analysis of HeLa cells using PP2C-α (D18C10) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：HeLa细胞，使用的抗体是PP2C-α (D18C10) XP® Rabbit mAb（蓝色），红色曲线表示非特异性阴性对照抗体。 | |
Western blot analysis of extracts from various cell lines using PP2C-α (D18C10) XP® Rabbit mAb.Western blot方法检测细胞提取物：各种细胞系。使用的抗体是PP2C-α (D18C10) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells using PP2C-α (D18C10) XP® Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red).激光共聚焦荧光法检测HeLa细胞，使用的抗体是PP2C-α (D18C10) XP® Rabbit mAb（呈绿色）。肌动蛋白丝被DY-554鬼笔环肽标记，呈红色。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PP2C-α (D18C10) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人乳腺癌组织。使用的抗体为PP2C-α (D18C10) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PP2C-α (D18C10) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人结肠癌组织。使用的抗体为PP2C-α (D18C10) XP® Rabbit mAb。 |
