| 货号 | 13048S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Rab11a, Rab11b, and Rab25 are members of the Rab11 subfamily of small Ras-like GTPases. Unlike universally expressed Rab11, typical Rab25 expression appears to be limited to gastrointestinal mucosa, kidney, and lung (1). Rab25 can associate with apical recycling vesicles to help regulate apical vesicle trafficking (2,3). Research studies indicate that atypical Rab25 expression can be associated with various forms of cancer. Increased Rab25 expression is associated with aggressive growth in ovarian and breast cancer, where Rab25 may inhibit apoptosis and promote cancer cell proliferation and invasion through regulation of vesicle transport and cellular motility (4-7). Interaction between Rab25 and β1 integrin promotes vesicle-mediated transport of integrin to pseudopodial tip membranes, fostering the persistent invasion of tumor cells (8). Conversely, the reported loss of Rab25 expression in a number of breast cancer cases has an unclear effect on cancer pathogenesis (9). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Confocal immunofluorescent analysis of MCF7 (positive, left) and HeLa (negative, right) cells using Rab25 (D4P6P) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Rab25 (D4P6P) XP® Rabbit mAb (绿).对MCF7(阳性，左)和HeLa(阴性，右)细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5®#4084 (fluorescent DNA dye) | |
Western blot analysis of extracts from various cell lines using Rab25 (D4P6P) XP® Rabbit mAb. 使用Rab25 (D4P6P) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from MCF7 (positive) and HeLa (negative) cells using Rab25 (D4P6P) XP® Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457 as a loading control.使用Rab25 (D4P6P) XP®Rabbit mAb对MCF7(阳性)和HeLa(阴性)细胞提取物进行western blot分析，β-Actin (D6A8) Rabbit mAb #8457作为上样量对照。 | |
Immunoprecipitation of Rab25 from ZR-75-1 cell extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or Rab25 (D4P6P) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Rab25 (D4P6P) XP® Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 Rab25 (D4P6P) XP® Rabbit mAb (lane 3)对ZR-75-1细胞中的Rab25进行免疫沉淀实验。Lane 1是10%上样量。使用Rab25 (D4P6P) XP® Rabbit mAb进行western blot分析。 |
