| 货号 | 6975S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Rab8 (D22D8) XP® Rabbit mAb recognizes endogenous levels of total Rab8 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring (1). Studies show that Rab8 is localized to the trans Golgi network (TGN), basolateral membrane, and vesicular structures where it helps regulate target protein transport between TGN and the basolateral membrane (1-3). Overexpression studies and mutation analysis of Rab8 and its associated Rab8GEF indicate additional roles in actin and microtubule remodeling during polarized membrane transport and membrane protrusion formation (4-6). Rab8 associates with myosin Vb and is required for translocation of GLUT4 following insulin stimulation in muscle (7,8). Control of target protein vesicle transport by Rab8 also regulates MT1-MMP activity during extracellular matrix formation and JRAB/MICAL-L2 at tight junction formation (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Confocal immunofluorescent analysis of MCF7 cells using Rab8 (D22D8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对MCF7细胞使用Rab8 (D22D8) XP® Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from various cell lines using Rab8 (D22D8) XP® Rabbit mAb. 对多种细胞系提取物使用Rab8 (D22D8) XP® Rabbit mAb兔单抗进行Western blot分析。 |
