| 货号 | 9040S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | DNA damage, if not repaired, can lead to genome instability and tumorigenesis. Eukaryotic cells use multiple (sometimes overlapping) signaling pathways to respond to agents that cause various types of DNA lesions. Downstream molecules in DNA repair pathways converge on the sites of DNA damage, resulting in cell cycle arrest and repair or apoptosis (1). Rad18 is an E3 ubiquitin ligase recruited to sites of DNA damage. Along with the E2 ubiquitin ligase Rad6, Rad18 is responsible for monoubiquitination of DNA damage proteins including the replication clamp PCNA and the Fanconi anemia core protein FANCD2. Monoubiquitination of these proteins signals to downstream effector molecules and results in the repair of either post-replication repair lesions via the translesion synthesis (TLS) pathway or DNA double strand breaks via homologous recombination (2-4). Phospho-proteomic studies indicate that Ser403 of Rad18 may be phosphorylated by ATM/ATR in response to DNA damage-inducing agents (5,6).DNA损伤如果没来得及修复，可能会导致基因组不稳定和肿瘤发生。真核细胞采用了多条（有时相互交叉）的信号通路对产生DNA损伤的物质进行反应。DNA修复通路的下游分子集中在DNA损伤的部位，引发细胞周期停滞和损伤或凋亡（1）。Rad18是一种聚集在DNA损伤部位的E3泛素连接酶。Rad18协同E2泛素连接酶Rad6，共同对DNA损伤蛋白进行单泛素化反应，这些蛋白包括复制钳PCNA和范可尼贫血核心蛋白FANCD2。这些蛋白的单泛素化将引发下游效应分子，以翻译合成（TLS）途径修复复制后损伤或以同源重组方式修复DNA双链损伤（2-4）。磷酸化蛋白质组学研究指出DNA损伤诱导试剂会诱导ATM/ATR磷酸化Rad18的403位丝氨酸（5,6）。 |
| 存放说明 | -20C |
| 计算分子量 | 80, 90 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), showing translocation of Rad18 from large spontaneous foci to numerous small damage-induced foci, using Rad18 (D2B8) XP® Rabbit mAb (green). Actin filaments were labeled with β-Actin (8H10D10) Mouse mAb #3700 (red).使用Rad18 (D2B8) XP® Rabbit mAb (绿色)对未处理（左）或UV处理（右）的HeLa细胞 进行激光共聚焦免疫荧光分析，以显示Rad18聚集到多个小的损失点。使用β-Actin (8H10D10) Mouse mAb #3700 (红色)标记肌动蛋白丝。 | |
Western blot analysis of extracts from various cell lines using Rad18 (D2B8) XP® Rabbit mAb.使用Rad18 (D2B8) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 |
