| 货号 | 5528S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IHC-P (1:1600) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from various cell lines using RSK2 (D21B2) XP® Rabbit mAb.Western blot方法检测细胞提取物：各种细胞系。使用的抗体是RSK2 (D21B2) XP® Rabbit mAb | |
Western blot analysis of recombinant GST-tagged RSK1, RSK2, and RSK3 (2 μg each) using RSK1 (D6D5) Rabbit mAb #8408 (left panel), RSK2 (D21B2) XP® Rabbit mAb #5528 (center panel), or GST (91G1) Rabbit mAb #2625 (right panel).Western blot方法检测：GST标记的重组RSK1, RSK2,RSK3 (分别2 μg)。使用的抗体是RSK1 (D6D5) Rabbit mAb #8408 (左侧), RSK2 (D21B2) XP® Rabbit mAb #5528 (中间)和GST (91G1) Rabbit mAb #2625 (右侧)。 | |
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with sodium arsenite (500 uM for 1 hr; right) using RSK2 (D21B2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Localization of RSK2 to "stress granules" in arsenite-treated cells is very similar to that seen by Eisinger-Mathason et. al. (Molecular Cell, Volume 1, 5 September 2008, Pages 722-736).激光共聚焦荧光法检测MCF7细胞，左侧样品未经处理，右侧样品用亚砷酸钠(500 uM 1 hr)处理。使用的抗体是RSK2 (D21B2) XP® Rabbit mAb（呈绿色）。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。亚砷酸盐处理的细胞中RSK2定位于"stress granules"，这个现象与Eisinger-Mathason 等人看到的现象十分相似。(Molecular Cell, Volume 1, 5 September 2008, Pages 722-736)。 | |
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with insulin, EGF, and serum (right), using RSK2 (D21B2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测MCF7细胞，左侧样品未经处理，右侧样品用胰岛素、EGF和血清处理。使用的抗体是RSK2 (D21B2) XP® Rabbit mAb（呈绿色）。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084）。 | |
Flow cytometric analysis of HeLa cells using RSK2 (D21B2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).Flow cytometric方法检测细胞提取物：HeLa细胞，使用的抗体是RSK2 (D21B2) XP® Rabbit mAb（蓝色），红色曲线表示使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using RSK2 (D21B2) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using RSK2 (D21B2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RSK2 (D21B2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using RSK2 (D21B2) XP® Rabbit mAb. |
