| 货号 | 5439S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | SKAR α/β (D65E8) XP® Rabbit mAb detects endogenous levels of total SKAR protein, α and β isoforms. By western blot, the antibody detects an approximately 21 kDa band of unknown origin in all primate cell types tested. |
| 使用方法 | WB(1:1000) IF-IC (1:3200) |
| 供应商 | CST |
| 背景 | p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3). |
| 存放说明 | -20C |
| 计算分子量 | 46, 43 |
Confocal immunofluorescent analysis of 293 cells using SKAR α/β (D65E8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用SKAR α/β (D65E8) Rabbit mAb兔单抗 (绿色)标记，共聚焦免疫荧光观察SKAR α/β (D65E8)蛋白在293细胞中定位。DY-554 phalloidin标记微丝蛋白（红色）。 | |
Western blot analysis of extracts from various cell types using SKAR α/β (D65E8) XP® Rabbit mAb. 使用SKAR α/β (D65E8) XP® Rabbit mAb兔单抗，Western blot分析不同细胞系中SKAR α/β (D65E8)蛋白水平。 |
