| 货号 | 3579S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:300) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
Western blot analysis of extracts from NTERA2 and NCCIT cells using Sox2 (D6D9) XP® Rabbit mAb.使用Sox2 (D6D9) XP® 兔单抗对NTERA2和NCCIT细胞提取物进行western blot分析。 | |
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Sox2 (D6D9) XP® Rabbit mAb.使用Sox2 (D6D9) XP® 兔单抗对HeLa细胞（蓝色）和NTERA2细胞（绿色）进行流式细胞分析。 | |
Confocal immunofluorescent analysis of NTERA2 (left) and HeLa (right) cells using Sox2 (D6D9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).使用Sox2 (D6D9) XP® 兔单抗(绿色)对NTERA2（左）和HeLa细胞（右）进行激光共聚焦荧光分析。肌动蛋白丝使用Alexa Fluor® 554 鬼笔环肽（红色）标记。 | |
Immunohistochemical anaylsis of paraffin-embedded NTERA2 cells (positive) (left) and HeLa cells (negative) (right) using Sox2 (D6D9) XP® Rabbit mAb.使用Sox2 (D6D9) XP® 兔单抗对石蜡包埋的NTERA2细胞（阳性）（左）和HeLa细胞（阴性）（右）进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus using Sox2 (D6D9) XP (TM) Rabbit mAb.使用Sox2 (D6D9) XP® 兔单抗对石蜡包埋的人食道鳞状上皮细胞癌组织进行免疫组化分析。 |
