| 货号 | 12774S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
Flow cytometric analysis of 293T cells, untransfected (blue) or transfected with a GFP/S-Tag construct (green), using S-Tag (D2K2V) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.用S-Tag (D2K2V) XP® Rabbit mAb进行293T细胞的流式检测，蓝色代表未转染细胞，绿色代表转染了GFP/S-Tag的细胞。二抗为Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414。 | |
Confocal immunofluorescent analysis of 293T cells transfected with a GFP/S-Tag construct using S-Tag (D2K2V) XP® Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用免疫荧光染色检测转染GFP/S-Tag的293T细胞，抗体用S-Tag (D2K2V) XP® Rabbit mAb (红色)。蓝色为DRAQ5® #4084 (fluorescent DNA dye)染色。 | |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with an S-Tag fusion protein, using S-Tag (D2K2V) XP® Rabbit mAb.用S-Tag (D2K2V) XP® Rabbit mAb及WB的方法检测未转染（-）或转染（+）S-Tag融合蛋白的293T细胞。 | |
Immunoprecipitation of transfected S-Tag fusion protein from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or S-Tag (D2K2V) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using S-Tag (D2K2V) XP® Rabbit mAb.在转染S-Tag融合蛋白的293T细胞提取物中做免疫共沉淀IP，lane 2是对照组， 用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 ；lane 3是实验组，用S-Tag (D2K2V) XP® Rabbit mAb。lane 1 是10%的input组。用S-Tag (D2K2V) XP® Rabbit mAb做WB。 |
