| 货号 | 8449S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | StAR (D10H12) XP® Rabbit mAb recognizes endogenous levels of total StAR protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Steroidogenic acute regulatory protein (StAR) plays a significant role in cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane (1). The 37 kDa precursor is cleaved to generate an active 28 kDa protein capable of facilitating cholesterol metabolism into pregnenolone (2,3). StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue (3). Abnormalities in StAR gene expression are impacted in autosomal Lipoid Congenial Adrenal Hyperplasia (LCAH) resulting in defects in pregnenolone and cortisol synthesis (4). The mechanism of cholesterol binding to StAR has yet to be elucidated (4). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Confocal immunofluorescent analysis of MLTC-1 cells (left) and C2C12 cells (right) using StAR (D10H12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测MLTC-1细胞(左)和C2C12细胞(右)提取物，蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084)。 | |
Western blot analysis of extracts from MLTC-1 cells and human, mouse, and rat adrenal tissue using StAR (D10H12) XP® Rabbit mAb. Wetern blot检测人，小鼠大鼠肾上腺MLTC-1细胞提取物，所用抗体为StAR (D10H12) XP® Rabbit mAb兔单抗。 |
