| 货号 | 9045S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Thymidylate Synthase (D5B3) XP® Rabbit mAb detects endogenous levels of total thymidylate synthase protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30 kDa subunits. TS is an intracellular enzyme that provides the sole de novo source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, investigators have concluded that TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Research studies have demonstrated that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). Researchers continue to investigate TS expression in different types of cancers (6-10). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
Western blot analysis of extracts from various cell lines using Thymidylate Synthase (D5B3) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb (lower) #8457. 对多种细胞系和组织抽提液使用Thymidylate Synthase (D5B3) XP? 兔单抗（上图）及β-Actin (D6A8)兔单抗#8457（下图）进行Western blot分析。 | |
Western blot analysis of extracts from SK-BR-3 and HCC827 cells, untreated or treated with 5-FU (1 μM) or lapatinib (1 μM) for 24 hr, using Thymidylate Synthase (D5B3) XP® Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). 对SK-BR-3和HCC827细胞，未处理或1uM 5-FU或1uM lapatinib处理24小时，抽提液使用Thymidylate Synthase (D5B3) XP? 兔单抗（上图）及β-Actin (13E5)兔单抗#4970（下图）进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Thymidylate Synthase (D5B3) XP® Rabbit mAb. 对石蜡包埋的人淋巴瘤使用Thymidylate Synthase (D5B3) XP? Rabbit mAb兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded SK-BR-3 cell pellets, untreated (left) or treated with LY294002 #9901 (right), using Thymidylate Synthase (D5B3) XP® Rabbit mAb. 对石蜡包埋的SK-BR-3细胞团，未处理（左）或LY294002 #9901处理（右），使用Thymidylate Synthase (D5B3) XP?兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Thymidylate Synthase (D5B3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人肺癌使用Thymidylate Synthase (D5B3) XP?兔单克隆抗体进行免疫组织化学分析，其中左图对照肽，右图抗原特异肽。 | |
Confocal immunofluorescent analysis of LN18 cells using Thymidylate Synthase (D5B3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对LN18细胞使用p75NTR (D4B3) XP?兔单抗（绿色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Flow cytometric analysis of HCC827 cells (blue) and HeLa cells (green) using Thymidylate Synthase (D5B3) XP® Rabbit mAb. 对HCC827（蓝）和Hela（绿）细胞使用Thymidylate Synthase (D5B3) XP?兔单抗进行流式细胞仪分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using Thymidylate Synthase (D5B3) XP® Rabbit mAb. |
