| 货号 | 4308S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Tuberin/TSC2 (D93F12) XP® Rabbit mAb detects endogenous levels of total tuberin protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:6400) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
Confocal immunofluorescent analysis of SH-SY5Y (left), wild-type MEF (middle) and TSC2-deficient (-/-) MEF cells (right) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析.SH-SY5Y（上图），野生型MEF（中图）和TSC2缺陷型(-/-)MEF细胞（下图），所使用的是Tuberin/TSC2 (D93F12) XP™ Rabbit mAb兔单抗（绿色）。肌动蛋白丝采用DY-554 phalloidin (red)标记。蓝色伪彩为DRAQ5® #4084 (荧光DNA 染料). | |
Western blot analysis of extracts from SH-SY5Y cells and rat brain using Tuberin/TSC2 (D93F12) XP® Rabbit mAb. Western blot 分析SH-SY5Y洗白和大鼠脑细胞，所用抗体为Tuberin/TSC2 (D93F12) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from wild-type (wt) and TSC2-deficient (-/-) mouse embryonic fibroblasts (MEF) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). Western blot 分析野生型（wt）和TSC2缺陷型(-/-)的小鼠的胚胎成纤维细胞(MEF），所使用的是Tuberin/TSC2 (D93F12) XP™ Rabbit mAb 兔单抗（上图）和Akt (pan) (C67E7) Rabbit mAb兔单抗 #4691 （下图）。 | |
Flow cytometric analysis of wild-type MEF (green) and TSC2-deficient (-/-) MEF cells (blue) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Flow cytometric analysis of wild-type MEF (green) and TSC2-deficient (-/-) cells (blue) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb (PE Conjugate). |
