| 货号 | 11896S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | UCHL1 (D8R2I) XP® Rabbit mAb recognizes endogenous levels of total UCHL1 protein. This antibody does not cross-react with other UCH family members. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the ubiquitin carboxy-terminal hydrolase (UCH) family of DUBs, which all possess a conserved catalytic UCH domain of about 230 amino acids. UCHL5 and BAP1 have unique, extended carboxy-terminal tails. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 expression is more widely distributed (3,4). Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Confocal immunofluorescent analysis of DU 145 (positive; left) and LNCaP (negative; right) cells using UCHL1 (D8R2I) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).Confocal immunofluorescent检测DU 145 细胞(阳性; 左) 和LNCaP细胞（阴性；右），采用UCHL1 (D8R2I) XP® Rabbit mAb（绿色）。内参照采用DY-554 phalloidin标记（红色）。 | |
Western blot analysis of extracts from various cell lines and rat brain tissue using UCHL1 (D8R2I) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).Western blot检测多种细胞提取物和大鼠大脑组织，采用UCHL1 (D8R2I) XP® Rabbit mAb (upper)或GAPDH (D16H11) XP® Rabbit mAb #5174 (lower)。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human UCHL1 (hUCHL1-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL3 (hUCHL3-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL5 (hUCHL5-Myc/DDK; +), and Myc/DDK-tagged full-length human BAP1 (hBAP1-Myc/DDK; +), using UCHL1 (D8R2I) XP® Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged人全长UCHL1 (hUCHL1-Myc/DDK; +)、Myc/DDK-tagged人全长UCHL3 (hUCHL3-Myc/DDK; +)、Myc/DDK-tagged人全长UCHL5 (hUCHL5-Myc/DDK; +)、Myc/DDK-tagged人全长BAP1 (hBAP1-Myc/DDK; +)的293T细胞，采用UCHL1 (D8R2I) XP® Rabbit mAb (upper)或者DYKDDDDK Tag Antibody #2368 (lower)。 |
