| 货号 | 88588S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human |
| 应用 | WB,IP,IHC,IF |
| 目标/特异性 | SQSTM1/p62 (D5L7G) Mouse mAb recognizes endogenous levels of total SQSTM1/p62 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) Immunohistochemistry (Paraffin) (1:800) Immunofluorescence (Immunocytochemistry) (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. |
| 存放说明 | -20C |
| 计算分子量 | 62 |
| 参考文献 | 1 . Kirkin, V. et al. (2009) Mol Cell 34, 259-69. 2 . Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9. 3 . Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23. 4 . Bjørkøy, G. et al. (2006) Autophagy 2, 138-9. 5 . Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5. 6 . Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80. 7 . Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6. 8 . Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7. 9 . Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9. 10 . Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14. 11 . Komatsu, M. et al. (2007) Cell 131, 1149-63. 12 . Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45. |
Western blot analysis of extracts from various cell lines using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extract from HCT 116 cells, untreated (-) or starved with Earles Basic Salt Solution (EBSS; 4 hr; +) using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot anlaysis of extracts from HeLa cells, untreated (-) or treated with Chloroquine #14774 (50 μM, overnight; +) using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SQSTM1/p62 siRNA I #6394 (+), or SignalSilence® SQSTM1/p62 siRNA II #6399 (+), using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of SQSTM1/p62 protein from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is SQSTM1/p62 (D5L7G) Mouse mAb. Western blot was performed using SQSTM1/p62 (D5L7G) Mouse mAb. A light chain-specific secondary antibody was used to avoid reactivity with heavy chain IgG. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SQSTM1/p62 (D5L7G) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SQSTM1/p62 (D5L7G) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using SQSTM1/p62 (D5L7G) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and Daudi (right) cell pellets using SQSTM1/p62 (D5L7G) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human inflamed gall bladder using SQSTM1/p62 (D5L7G) Mouse mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with chloroquine (50 μM overnight; right), using SQSTM1/p62 (D5L7G) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
