| 货号 | 95394S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC |
| 目标/特异性 | Rab27A (D7V6B) Rabbit mAb recognizes endogenous levels of total human Rab27A protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rab27 is a member of the Ras superfamily of small Rab GTPases implicated in exocytosis (1-2). The protein is localized in secretory lysosomes, such as melanosomes in melanocyte or lytic granules in cytotoxic T cells to control exosome secretion pathway (3-5). Rab27 has two isoforms, Rab27a and Rab27b. Rab27a colocalizes with part of CD63 staining vesicles, and Rab27b shows perinuclear distribution. Target knock out studies indicate that the isoforms control different steps of the exosome secretion pathway (6). Rab27a interacts with a wide range of effectors and is involved in multiple steps of exocytosis depending on the effector it associated with and the cell type that is involved (1,2). Rab27a has been shown to be an important player in leukocyte function, cancer metastasis and invasion, and insulin secretion (7-11) |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | 1 . Fukuda, M. (2013) Traffic 14, 949-63. 2 . Izumi, T. (2007) Endocr J 54, 649-57. 3 . Elstak, E.D. et al. (2011) Blood 118, 1570-8. 4 . Bahadoran, P. et al. (2001) J Cell Biol 152, 843-50. 5 . Wood, S.M. et al. (2009) Blood 114, 4117-27. 6 . Ostrowski, M. et al. (2010) Nat Cell Biol 12, 19-30; sup pp 1-13. 7 . Catz, S.D. (2013) J Leukoc Biol 94, 613-22. 8 . Herrero-Turrión, M.J. et al. (2008) J Immunol 181, 3793-803. 9 . Ostenfeld, M.S. et al. (2014) Cancer Res 74, 5758-71. 10 . Dong, W. et al. (2015) Discov Med 20, 357-67. 11 . Cazares, V.A. et al. (2014) Traffic 15, 997-1015. |
Confocal immunofluorescent analysis of SK-MEL-28 (left) and Raji (right) cells using Rab27A (D7V6B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of Rab27A protein from SK-MEL-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Rab27A (D7V6B) Rabbit mAb. Western blot analysis was performed using Rab27A (D7V6B) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Rab27A (D7V6B) Rabbit mAb. |
