| 货号 | 95357S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC F |
| 目标/特异性 | HSP27 (D6W5V) Rabbit mAb recognizes endogenous levels of total HSP27 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | 1 . Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373. 2 . Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803. 3 . Rouse, J. et al. (1994) Cell 78, 1027-1037. 4 . Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956. 5 . Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214. 6 . Rousseau, S. et al. (1997) Oncogene 15, 2169-2177. |
Confocal immunofluorescent analysis of HeLa (left) and HT-1080 (right) cells using HSP27 (D6W5V) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of HT1080 cells (blue) and HeLa cells (green) using HSP27 (D6W5V) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using HSP27 (D6W5V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of HSP27 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HSP27 (D6W5V) Rabbit mAb. Western blot was performed using HSP27 (G31) Mouse mAb #2402. |
